This study investigated the protective effects of Sargassum sagamianum extract (SSE) on INS-1 pancreatic β cells against high glucose-induced oxidative stress and apoptosis. Treatment with glucose at high concentrations (30 mM)… Click to show full abstract
This study investigated the protective effects of Sargassum sagamianum extract (SSE) on INS-1 pancreatic β cells against high glucose-induced oxidative stress and apoptosis. Treatment with glucose at high concentrations (30 mM) caused β cell apoptosis, whereas treatment with SSE protected the β cells from high glucose-induced damage, by recovering the cell viability. Treatment with SSE at concentrations of 10–100 μg/mL decreased lipid peroxidation and intracellular reactive oxygen species and nitric oxide levels, and increased cell viability and insulin secretion in high glucose pretreated INS-1 cells in a dose-dependent manner. Moreover, SSE treatment significantly reduced the expression of pro-apoptotic Bax, cytochrome c, caspase-3, and caspase-9, while the expression of anti-apoptotic Bcl-2 increased. The type of cell death was examined by annexin V/propidium iodide staining, which revealed that SSE treatment markedly reduced high glucose-induced apoptosis. These findings suggest that SSE could be useful as a functional food, protecting pancreatic β cells against high glucose-induced oxidative stress and apoptosis.
               
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