LAUSR

Non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino), an important vegetable in East Asian countries, is rich in vitamin C, dietary fiber, and other health-enhancing factors. As a staple vegetable, its genetic studies have been hindered due to the lack of polymorphic genetic markers. In this research, 2.3 and 2.1 Gb RNA-seq data have been obtained for a heat-sensitive cultivar “GHA” and a heat-resistant cultivar “XK”, respectively. Totally, 3267 novel transcripts were identified from the assembled 29,037 transcripts. A total of 139 differential expressed genes (DEGs) have been identified. Most DEGs were involved in the signal transduction and general function prediction. Moreover, 37 polymorphic EST-SSR loci and 285,573 SNP markers have been developed. This study will provide valuable transcript sequences and functional markers for further genetic diversity analysis, quantitative trait locus identification, as well as molecular marker assisted selection of Brassicaceae species.