LAUSR

A facile one-pot method was used to fabricate highly dispersed silver and gold nanoparticles attached to the surface of reduced graphene oxide (Ag–Au/rGO) nanohybrids via in situ incorporation of metal ions and chitosan-induced reduction. Chitosan species have a dual function in the synthesis route where they simultaneously stabilize the rGO nanosheets, furthermore Ag and Au nanoparticles nucleation as well as its reducing function. The crystal structure and morphology of the as-synthesized samples have been characterized, revealing the formation of spherical Ag and Au nanoparticles with FCC crystal structure attached to the surface of rGO nanosheets. The as-synthesized nanocomposites have shown a linear response for glucose sensing in the range from 0.25 to 25 mM with a detection limit close to 53 µM. This gluco-sensor exhibited high performance in detecting the released current in the human serum. Therefore, the results open a new route of using Ag–Au/RGO nanohybrids as a promising electrochemical biosensor.