LAUSR

Enzymatic hydrolysis is commonly used to produce bioactive peptides from protein such as casein that is rich in bioactive peptide sequences. In this study, a Bacillus metalloendopeptidase (PROTIN SD-NY10) was used to hydrolyze casein to produce bioactive peptides with antidiabetic and antioxidant activities. Several factors affecting the hydrolysis were assessed such as hydrolysis time, metalloendopeptidase concentration, casein concentration, pH, and temperature. The results showed that casein could be effectively hydrolyzed by the metalloendopeptidase, and the casein hydrolysates were shown with high inhibitory activities on α-glucosidase and α-amylase, as well as high antioxidant activity. The high inhibitory activities on α-glucosidase (68.5–80.55%) and α-amylase (74.10–77.15%) were obtained under the selected hydrolysis conditions (pH 7.5, 40 °C, 1% metalloendopeptidase, 10% casein, and 3 h). Meanwhile, the high DPPH radical scavenging activity (55.02–69.40%) could also be obtained under the same hydrolysis conditions. Therefore, the casein hydrolysate obtained by hydrolysis with a metalloendopeptidase of this study could be explored as a bioactive agent for potential application in functional products.