LAUSR

Background Presented work studies the association of COVID-19 severity, patient demographics, and clinical history with cycle threshold (Ct) values of SARS CoV2-rRT-PCR. We studied the Ct values for Orf1ab, N, and RdRp genes in association with all the factors mentioned above. Methods and results We examined the individuals (n = 6331) that consulted two private diagnostic centers for COVID-19 testing. SARS‐CoV‐2 was detected by RT‐PCR assays using different commercial kits. Clinical and demographic information was collected by the attending health care professional. Ct values were not associated with the age, sex, or clinical history of the patient. Orf1ab and N genes Ct values were only weakly associated with symptoms at the time of the SARS-CoV-2 RT-PCR test. Also, the distributions of Ct values in SARS-CoV-2 positive patients are very similar irrespective of symptomatology. Conclusion We conclude that the Ct values may have limitations in reliably predicting COVID-19 severity and should be used or reported with caution. Supplementary Information The online version contains supplementary material available at 10.1007/s11033-022-07360-x.