LAUSR

The objective of this study is to investigate the bactericidal efficiency of atmospheric pressure non-thermal (cold) dielectric barrier discharge plasma on biofilms of Staphylococcus aureus and Escherichia coli. In general, cold plasma is a mixture of electrons, ions, neutral atoms and molecules. The different particles in cold plasmas exhibit different energies, i.e. electrons are much more energetic than other particles. This feature of cold plasmas allows to produce chemically reactive species at near room temperature that can be used in biological applications. Bacteria inactivation was performed using the air direct plasma (with reactive species, UV light, excited species and electric fields). Discharge power density during the experiment was equal to 70 mW/cm2 and plasma dose was regulated by the treatment time. Bacterial biofilms were treated with the non-thermal plasma for 10–300 s. The most effective reduction in the number of S. aureus cells was found after 300 s of treatment and was 2.77 log10 that is 99.83%. When the biofilm of E. coli was used in the experiment, killing of bacteria was independent of the exposure time and the mortality of cells did not exceed 0.48 that is 66.7% kill. The lethal effect on E. coli and S. aureus cells were observed after 300 and 120 s of plasma treatment, respectively but it was necessary to remove the layers of dead cells. The proposed process of removing dead cell layers was carried out due to the probable shielding effect, i.e. dead cells prevent further penetration of active plasma species into the deeper layers of the biofilm. It was shown that the effectiveness of cell destruction by the non-thermal plasma depends on the thickness of biofilm.