LAUSR

Platelet concentrates (PC) are affected by biochemical, morphological and functional changes during storage that are named platelet storage lesion (PSL) can decrease the survival and quality of platelets. The potential role and importance of the microRNAs in diagnosis of this process could be remarkable. The aim of this study was to determine miR-145 and miR-326 associated with apoptosis during PC storage. Ten PC prepared by platelet-rich plasma method were selected by simple random sampling in Iranian Blood Transfusion Organization. The expression pattern of miR-326 and miR-145 during PC storage for 7 days was determined by quantitative Real-time RT-PCR, and then the relationship between expression levels of both microRNAs and some PC quality markers was investigated. Glucose concentration, cell viability, platelet count and aggregation during storage showed a significant decrease compared to day zero (P < 0.05). Reduction of these variables showed a direct correlation with a significant decreasing trend in expression level of miR-145 (r = 0.94, 0.98, 0.96 and r = 0.99, P = 0.01). Lactate dehydrogenase activity showed a significant increase during platelet storage compared to day zero (P < 0.05), which was directly correlated with increased expression of miR-326 (r = 0.97, P = 0.01). Changes in the expression pattern of miR-326 and miR-145 along with biochemical markers, all indicate a decrease in platelet quality during 7 days of storage. Due to the significant correlation between the expression changes of miR-326 and miR-145 with PC quality markers, it seems both of these microRNAs can be considered as remarkable markers for early diagnosis of the PC quality during storage.