Photo by sonika_agarwal from unsplash
We report the development of a stem cell culture system using surface functionalized polymeric micropillars with tunable interfacial chemical and physical properties. Micropillars, modified with peptides derived from vitronectin, can… Click to show full abstract
We report the development of a stem cell culture system using surface functionalized polymeric micropillars with tunable interfacial chemical and physical properties. Micropillars, modified with peptides derived from vitronectin, can be used as defined substrates to maintain both human embryonic stem cells and human induced pluripotent stem cells for up to 3 months without differentiation. The surface chemical properties of the micropillars were more critical than the physical properties in maintaining stem cells. However, the physical properties of the micropillars influenced the stem cell fate. Softer micropillars promoted the undifferentiated state, and more rigid micropillars encouraged stem cell differentiation.
Journal Title: Topics in Catalysis
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!