LAUSR

Pichia kudriavzevii YB5, mutated from Pichia kudriavzevii A16 with a strong ability to remove cadmium ions, was immobilized by polyurethane foam and alginate gels in this work. The immobilization conditions were optimized as follows: sodium alginate concentration of 2% (w/v), calcium chloride concentration of 2% (w/v), biomass dose of 1 × 109 cell/mL, and cross-linking time for 4 h. Then, the results of batch adsorption experiments showed that the removal capacity of prepared bioadsorbent was significantly affected by the pH of media, contact time, and the initial Cd(II) concentration, and a suitable adsorption conditions of Cd(II) could be achieved with a pH value of 6.0 at 20 °C for 90 min. Kinetic and isothermal results indicated the behavior of Cd(II) adsorption onto immobilized P. kudriavzevii YB5 fitted to the pseudo-second-order kinetic equation and the Langmuir adsorption model. Thermodynamic results showed that the Cd(II) adsorption process was endothermic and spontaneous in nature. Besides, the Cd(II) removing capacity of the prepared bioadsorbent was also tested in the oyster hydrolysates, showing an average removal rate of 54.35%. Thus, the immobilized P. kudriavzevii YB5 adsorbent had great potential for application in aquatic products to ensure the food safety.