LAUSR

Microbial quality of irrigation waters is a substantial food safety factor. Escherichia coli (E. coli) and Enterococci are used as the fecal indicator bacteria (FIB) to assess microbial water quality. Analysis of temporally stable patterns of FIB can facilitate effective monitoring of microbial water quality. The objectives of this study were (1) to investigate the spatiotemporal variation of E. coli and Enterococci concentrations in a large creek traversing diverse land use areas and (2) to explore the presence of temporally stable FIB concentration patterns along the creek. Concentrations of both FIB were measured weekly at five water monitoring locations along the 20-km long creek reach in Pennsylvania at baseflow for three years. The temporal stability was assessed using mean relative deviations of logarithms of FIB concentration from the average across the reach measured at the same time. The Spearman rank correlation coefficients between logarithms of FIB concentrations on consecutive sampling times was another metric used to assess the temporal stability of FIB concentration patterns. Logarithms of FIB concentrations had sinusoidal dependence on time and significantly correlated with temperature at all locations Both FIB exhibited temporal stability of concentrations. The two most downstream locations in urbanized areas tended to have logarithms of concentrations higher than the average along the observation reach. The location in the upstream forested area had mostly lower concentrations (log E. coli 1.59, log Enterococci 1.69) than average (log E. coli 2.07, log Enterococci 2.20). concentrations in colony-forming units (CFU) (100 mL)−1. Two locations in the agricultural and sparsely urbanized area had these logarithm values close to the average. The temporal stability was more pronounced in cold seasons than in warm seasons. No significant difference was found between pattern determined for each of three observation years and for the entire three-year observation period. The Spearman rank correlations between observations on consecutive dates showed moderate to very strong relationships in most cases. Existence of the temporal stability of FIB concentrations in the creek indicates locations that inform about the average logarithm of concentrations or the geometric mean concentrations along the entire observation reach.