LAUSR

This study was conducted to investigate the adverse effects of cadmium (Cd) on the production performance, serum biochemistry, liver antioxidant status, histopathology, and egg residue in laying hens. A total of 72 healthy Hy-Line brown laying hens at 40-week-old were randomly assigned to four diets containing 0 (control diet), 15, 30, or 60 mg/kg Cd for 6 weeks. Laying hens exposed to 60 mg/kg Cd had lower egg production rate and worse feed to egg ratio ( P < 0.05). Dietary Cd exposure (≥ 15 mg/kg) significantly decreased hepatic glutathione peroxide (GPX) activities, while increasing malondialdehyde (MDA) ( P < 0.05). Hepatic histopathology and ultrastructure also showed damage and the symptoms were exacerbated in a dose-dependent manner. The residue of Cd in the yolk was increased with increasing dietary Cd concentration. The mRNA expression levels of mt4L, mt3, sod1, sod2, gpx1, gpx3 , and gpx4 in the liver of laying hens exposed to 60 mg Cd/kg feed were significantly decreased ( P < 0.05). In conclusion, dietary Cd exposure at ≥ 15 mg/kg induced hepatic damage in laying hens, indicating that the content of Cd in feed must be critically controlled.