LAUSR

To determine the feasibility of enhancing co-conversion of endogenous C and N in dairy wastewater by surfactants, the effects of varying dosages of sodium lignosulfonate (LS), sodium dodecyl sulfate (SDS), and Tween-80 on mesophilic hydrolytic acidification coupled microalgae culture system were investigated. Tween-80 had a positive effect on hydrolytic acidification, while LS had no clear effect and SDS had a negative effect. Tween-80 significantly increased the C/N ratio in hydrolytic acidification liquor (HAL) (maximum HAc/NH4+-N rate reached 7.90 in 2.9% Tween-80 test). Pyrosequencing analysis demonstrated that community richness and diversity decreased and the proportion of acidobacteria increased with increasing Tween-80 dosage. Furthermore, the effect of Tween-80-enhanced HAL on microalgae (Chlorella pyrenoidosa) growth and nitrogen removal and the assimilation of C and N on the microalgae culture system were investigated. The biomass concentration and a C and N assimilation yield of 4.8% Tween-80 test (1.75 g/L, 825.01 mg/L, 126.68 mg/L) were significantly higher than those of both the low-concentration Tween-80 tests and control. The yield of protein and carbohydrates and higher heating value in the microalgae culture system were also promoted by Tween-80.