LAUSR

Heavy metal and metalloid pollution is a matter of concern in animal, human and environmental health (One Health) and also in wildlife conservation worldwide. Studying wild mammals in toxicology has been contributing significantly to our knowledge, namely to find out the most critical regions, to understand bioaccumulation and biomagnification phenomena or to evaluate their toxic effects. However, not all the animal tissues and organs provide the same information or should be interpreted in the same way. The best sample to use will depend on the objectives and conditions of the study. This review aims to compare invasive and non-invasive samples to biomonitor heavy metals, providing a brief resume of their advantages, limitations and examples of use. Further research, using a wider range of mammalian species, is required to establish what information can be obtained in biomonitoring studies that use non-invasive samples (such as hair, faeces and parasites) and/or invasive samples (such as blood, liver, kidney, bone and other organs).