LAUSR

The objective of this work was to investigate antifungal actions of glycinin basic peptide (GBP), a natural preservative derived from soybean, against Aspergillus niger (A. niger). GBP exhibited the antifungal activity against A. niger with minimum inhibitory concentration of 1.5 mg/mL. The analysis of FSC and SSC manifested that GBP treatment could cause A. niger cells to shrink to become small and their granularity to complicate. And observations of electron microscopy directly showed more than 1.5 mg/mL GBP obviously destructed membrane, morphology, and organelles of mycelia and spores. The K+, Ca2+, and Mg2+ leakage further verified the disruptive region of GBP was membrane of A. niger. Moreover, the mitochondrial dysfunction by GBP was elucidated by the increase of intracellular reactive oxygen species and decrease of mitochondrial membrane potential. Taken together, GBP induced cellular damage and death of A. niger by collaborative action on cell membrane and mitochondria.