Glucose oxidase (GOx) was immobilized on vanadium pentoxide xerogel (V2O5) onto a carbon-based screen-printed electrode to evaluate the possibility of constructing a V2O5/GOx biosensor. Structural characterizations of V2O5/GOx demonstrated a… Click to show full abstract
Glucose oxidase (GOx) was immobilized on vanadium pentoxide xerogel (V2O5) onto a carbon-based screen-printed electrode to evaluate the possibility of constructing a V2O5/GOx biosensor. Structural characterizations of V2O5/GOx demonstrated a weak interaction between the species. Cyclic voltammetry results for V2O5/GOx after immobilization in 4.4 mmol/L of glucose, pH 5, in PBS medium indicated a stability tendency after several cycles and also an increase of total charge. The use of an electrochemical quartz crystal microbalance technique evidenced a mass loss after several cycles. Experimental studies using variations of glucose concentration and electrical characterization by extended gate field effect transistors is also discussed.
               
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