LAUSR

Bark beetles Tomicus yunnanensis and T. minor are two important pests of Pinus yunnanensis and can cause massive death of pine trees. In this study, we examined several traits related to photosynthesis in P. yunnanensis and their relationship with antibiotic defense responses after joint attack by the two bark beetles at the shoot and the trunk stages. When shoots were attacked by the beetles, the abundance of chlorophylls, carotenoids, and the rates of net photosynthesis (Pn) and transpiration (E) decreased in needles, while the levels of superoxide dismutase and malondialdehyde remained unchanged in both needles and phloem. The activity of peroxidases also remained unchanged in needles, but increased in phloem. The activity of catalases increased in both needles and phloem. When trunks were attacked by the bark beetles, chlorophyll abundance, Pn, E, and antioxidative enzyme activities all declined, and the declines were more pronounced than in the attacked shoots. A decrease in protein concentrations was also observed in needles and phloem from the attacked pines. Attack on shoots by the bark beetles suppressed host defense and provided a favorable environment for larval growth and development, resulting in long-term decline of pine growth potential. The results suggest that attacks on trunks by beetles caused more severe damage to host trees than attacks on shoots.