LAUSR

In this study, the toxic effects of nickel oxide nanoparticles (NiO-NPs) on the model organism Mediterranean mussel (Mytilus galloprovincialis) gill, digestive gland, and hemolymph tissues for 96 h were investigated. Lipid peroxidation (MDA) determination was performed to reveal the oxidative stress generation potential of nanoparticles, and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) enzyme levels were measured to determine antioxidant responses. Lysosomal membrane stability and total hemocyte counts were performed to determine cytotoxic effects. All parameters were altered in different concentrations of NiO-NPs (2, 20, and 200 mg L-1). The SOD levels increased depending on the concentration (p < 0.05), and the increases in CAT, GPx, and GST levels were lower at 20 mg L-1 concentration (p < 0.05). There was a slight difference between the exposure and the control groups in terms of GR enzyme. The MDA level increased in parallel with the concentration (p < 0.05), the stability of the cell membrane (p < 0.05), and the number of hemocyte cells decreased as a result of exposure (p < 0.05). The results emphasize that NiO-NPs may have negative effects on the aquatic environment.