LAUSR

In this study, thin layer chromatography (TLC) coupled with visible (Vis) spectrophotometry was established as a simple method of determining diacylglycerols (DGs). Samples were analyzed on TLC plates, developed, and then eluted to prepare the test solution. This solution was scanned on an ultraviolet spectrophotometer to ensure that the maximum absorbance of DGs was at 700 nm. Afterward, the relationship between the absorbance and the DG solution was investigated at different concentrations. The linearity of the calibration was within 10–90 mg/mL. The method was highly accurate, as evidenced by its percent recovery of 98.66%. Its slightly low precision can meet the measurement requirements through repeated determination. Moreover, color development was stable enough within 1 h, and the detection limit was 0.51 mg/mL. Therefore, TLC coupled with Vis spectrophotometry could be used to determine DG contents within a certain range.