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Molecular Enrichment for Qualitative Molecular Pathogen Detection in Food

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Reliable detection of low levels of pathogenic microbial contaminants is one of the most important prerequisites to guarantee safe food production. In this work, the use of molecular enrichment (ME)… Click to show full abstract

Reliable detection of low levels of pathogenic microbial contaminants is one of the most important prerequisites to guarantee safe food production. In this work, the use of molecular enrichment (ME) subsequent to the sample preparation method Matrix-Lysis was evaluated solely for qualitative molecular pathogen detection from complex matrices. It could be successfully shown that the LOD of an all-molecular pathogen detection approach for food was effectively lowered by a factor of ten from 1.60 × 101 CFU/ml to 1.60 × 100 CFU/ml due to ME.

Keywords: detection; molecular enrichment; molecular pathogen; pathogen detection; food

Journal Title: Food Analytical Methods
Year Published: 2017

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