LAUSR

To monitor monensin (MON) residues in chicken tissues, a monoclonal antibody (mAb)–based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed in this study. The haptenMON was obtained by adding hydrochloric acid to a MON salt, and then was coupled with carrier protein. After the inoculation of female Balb/c mice and cell fusions, one cell line, MON/4C9, was obtained. The MON/4C9 antibody exhibited the ability to specifically recognize MON with IC50 1.28 μg L−1.Based on this mAb, an optimized ic-ELISA protocol was performed using only methanol-water (8:2, v/v) in chicken tissue samples. The limits of detection and the limits of quantification of MON in various sample matrices varied from 0.38 to 1.01 μg kg−1. The recoveries ranged from 71.9 to 116.9% in chicken tissues, and the intra- and inter-assay CVs were all less than 18%. Moreover, the developed method also exhibited a positive correlation with the results of HPLC-MS conducted on the samples. These results suggest that the prepared mAb and the developed ic-ELISA method will be a useful tool for detecting MON in chicken tissues.