LAUSR

Two unknown Gram-stain-positive, catalase- and oxidasenegative, non-motile, and coccus-shaped bacteria, designated MN-17 T and MN-09, were isolated from yaks faeces (Bos grunniens) in the Qinghai-Tibet Plateau of China. 16S rRNA gene sequence-based comparative analyses revealed that the two strains were grouped within the genus Vagococcus , displaying the highest similarity with Vagococcus xieshaowenii CGMCC 1.16436 T (98.6%) and Vagococcus elongatus CCUG 51432 T (96.4%). Both strains grew optimally at 37°C and pH 7.0 in the presence of 0.5% (w/v) NaCl. The complete genome of MN-17 T comprises 2,085 putative genes with a total of 2,190,262 bp and an average G + C content of 36.7 mol%. The major fatty acids were C 16:0 (31.2%), C 14:0 (28.5%), and C 18:1 ω 9 c (13.0%); the predominant respiratory quinone was MK-7 (68.8%); the peptidoglycan type was A4α( l -Lys- d -Asp); and the major polar lipid was diphosphatidylglycerol. Together, these supported the affiliation of strain MN-17 T to the genus Vagococcus. In silico DNA-DNA hybridization and the average nucleotide identity values between MN-17 T and all recognized species in the genus were 21.6–26.1% and 70.7–83.0%, respectively. MN-17 T produced acid from d -cellobiose, d -fructose, glycerol, d -glucose, N-acetyl-glucosamine, gentiobiose, d -mannose, d -maltose, d -ribose, d -saccharose, salicin, d -trehalose, and d -xylose. These results distinguished MN-17 T and MN-09 from closely related species in Vagococcus . Thus, we propose that strains MN-17 T and MN-09 represent a novel species in the genus Vagococcus , with the name Vagococcus zengguangii sp. The type strain is MN-17 T (= CGMCC 1.16726 T = GDMCC 1.1589 T = JCM 33478 T ).