LAUSR

Procalcitonin (PCT), C-reactive protein (CRP), and white blood cell (WBC) have been used as markers of bacterial infection in children for decades. Previous studies have suggested PCT, CRP, WBC, and percentage of neutrophils (%N) may be useful in detecting bacterial infection in children [1–4]. However, elevated levels of these biomarkers have also been noted in children with enterovirus infection [5–7]. In a study involving 5692 hospitalized children with herpangina or hand, foot, and mouth disease (HFMD) in two periods of years, the medians of CRP were 50.1 and 42.5 mg/L, respectively; and the medians of WBC were 14.1 and 15.3 × 109/L, respectively [5]. These biomarkers were sometimes considered as evidence of bacterial coinfection in children with enterovirus infection, which resulted in a high antibiotic prescribing rate. For children hospitalized for HFMD, the antibiotic prescribing rates ranged from 7.4% to 100% in previous studies [5, 8, 9]. However, the value of these biomarkers in detecting bacterial coinfection among children with enterovirus infection is unclear. We conducted a retrospective study in Shenzhen Children’s Hospital, a 1300-bed tertiary care facility in Shenzhen, China. The study population consisted of all children hospitalized for herpangina or HFMD between January 2015 and December 2020. Enterovirus infection was defined as the presence of a positive polymerase chain reaction (PCR) test for enterovirus with an oropharyngeal swab or stool specimens. Single enterovirus infection was defined as the presence of enterovirus infection which could fully explain all the symptoms of the patient. Enterovirus infection severity was classified as mild or severe based on the Chinese guideline for the diagnosis and treatment of HFMD (2018 edition) [10]. Definitions of bacterial coinfection diseases are summarized in Table 1. Cases were defined as patients with enteroviral and bacterial coinfection disease. Two controls with a single enterovirus infection were matched to each case by age (days) and sex. For cases who could not be matched by exactly the same age, they would be matched with controls of the most similar age. Patients with any of the following factors were excluded: negative or absence of PCR test for enterovirus; absence of both the CRP and PCT tests; comorbidity other than bacterial coinfection; liver dysfunction (prothrombin time > 18 seconds and serum bilirubin ≥ 20 μmol/L) [12]; immunocompromised state or immunodeficiency; underlying chronic disease (autoimmune disease, thyroid disease, malnutrition, congenital heart disease, and chronic lung disease). The clinical variables were measured every day during hospitalization. Blood samples were collected during hospitalization as needed to guide management decisions. Categorical variables were presented as number and percentage. Continuous variables were presented as mean ± standard deviation (SD) if they were normally distributed or median (25–75% interquartile range) if they had a skewed distribution. Chi-square test was used for categorical variables. The Student t test or Mann–Whitney test was used for continuous variables, as appropriate. Binary logistic regression analysis was also performed to control confounding effects. Data analysis was performed by SPSS 26.0 software. All P-values were two-tailed, and P < 0.05 was considered to indicate statistical significance. We identified 45 cases and 90 controls (Fig. 1, Table 2). CBC and CRP tests were performed in all the included children. PCT test was performed in 37 cases and 83 controls. The medians of test timing (days after fever onset) for PCT, CRP, WBC, and N% were, respectively, 4, 4, 3, 3 in cases and 3, 2, 2, 2 in controls. The maximal levels of inflammatory biomarkers in cases were as follows: PCT, 6.78 ng/mL; CRP, 135.1 mg/L; WBC, 32.53 × 109/L; and %N, 88.4%. * Ji-Kui Deng [email protected]