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We describe novel hybrid primers for the successful amplification and sequencing of the mitochondrial cytochrome b (cytb) gene in the family Siganidae. We aligned 403 cytb sequences from 29 species… Click to show full abstract
We describe novel hybrid primers for the successful amplification and sequencing of the mitochondrial cytochrome b (cytb) gene in the family Siganidae. We aligned 403 cytb sequences from 29 species and found multiple potential conserved hybrid primers (< twofold degeneracy). The best set was selected, and successfully amplified and sequenced ~ 1100 bp of cytb in the Siganidae.
Keywords:
hybrid primers;
sequencing mitochondrial;
mitochondrial cytochrome;
primers sequencing;
gene;
novel hybrid
Journal Title: Conservation Genetics Resources
Year Published: 2019
Link to full text (if available)
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Journal Title: Conservation Genetics Resources
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!