LAUSR

Abstract Investigation of the entomogenous fungus Setosphaeria rostrate LGWB-10 from Harmonia axyridis led to the isolation of four new isocoumarin derivatives, setosphlides A–D ( 1 – 4 ), and four known analogues ( 5 – 8 ). Their planar structures and the relative configurations were elucidated by comprehensive spectroscopic methods. The absolute configurations of isocoumarin nucleus for 1 – 4 were elucidated by their ECD spectra. The C-10 relative configurations for the pair of C-10 epimers ( 1 and 2 ) were established by comparing the magnitude of the computed 13 C NMR chemical shifts (Δ δ calcd. ) with the experimental 13 C NMR values (Δ δ exp. ) for the epimers. All of the isolated compounds ( 1 – 8 ) were evaluated for their cytotoxicities against four human tumor cell lines MCF-7, MGC-803, HeLa, and Huh-7. Graphic Abstract