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The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples

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Detection limits of PCR to detect Cryptosporidium spp. were determined in ninety-six preserved fecal samples (obtained for a different project). Small subunit ribosomal RNA gene was amplified from each sample.… Click to show full abstract

Detection limits of PCR to detect Cryptosporidium spp. were determined in ninety-six preserved fecal samples (obtained for a different project). Small subunit ribosomal RNA gene was amplified from each sample. The best detection limit of the PCR method was > 150 oocysts.

Keywords: fecal samples; cryptosporidium spp; detection limit; limit pcr

Journal Title: National Academy Science Letters
Year Published: 2019

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