LAUSR

The objective of the study was to find out odorant-binding protein 2 and salivary protein MP58 as potential RNAi targets in Myzus persicae . These potential targets were confirmed by reverse transcriptase polymerase chain reaction. Genes were sequenced and GenBank IDs allotted were MN183129 for odorant-binding protein 2 and MN183130 for MP58. ERNAi tool designed more than 250 siRNA targets from which seven were mentioned with structural map positions for both genes without any off-target. Maximum mortality was observed with odorant-binding protein 2 (70%); followed by MP58 (57%) in comparison to $$\underline{\mathbf{+ve}}$$ + ve ̲ and $$\underline{\mathbf{-ve}}$$ - ve ̲ control 8d after dsRNA feeding (15 ng/μL). Quantitative polymerase chain reaction expressed the reduced expression of odorant-binding protein 2 (33%) followed by MP58 (40%) gene 8Ds after dsRNA feeding in comparison to control (98%). Phylogenetic analysis exhibited homology of M. persicae odorant-binding protein 2 to grain, oat, pea and corn aphid and MP58 with pea and corn aphid; broaden its application for main staple crops and more specifically for M. persicae .