LAUSR

Increased consumption of raw and par-boiled rice results in the formation of methylglyoxal (MG) at higher concentration and leads to complications in diabetic patients. Highly sensitive electrochemical biosensor was developed using glutathione (GSH) as a co-factor with vanadium pentoxide (V2O5) as a nano-interface for MG detection in rice samples. The Pt/V2O5/GSH/Chitosan bioelectrode displayed two well-defined redox peaks in its cyclic voltammograms for MG reduction. This occurred as two electron transfer process where MG gained two electrons from oxidized glutathione disulfide and formed hemithioacetal. The current density response of the fabricated bioelectrode was linear towards MG in the concentration range of 0.1-100 μM with the correlation coefficient of 0.99, sensitivity of 1130.86 μA cm-2 μM-1, limit of detection of 2 nM and response time of less than 18 s. The developed bioelectrode was used for the detection of MG in raw and par-boiled rice samples.