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Existing methodologies for detecting Pseudouridine (Ψ) mostly use CMCT labeling or radiolabeling. Described herein is a sensitive and quantitative method for Ψ detection that does not need this labelling. This… Click to show full abstract
Existing methodologies for detecting Pseudouridine (Ψ) mostly use CMCT labeling or radiolabeling. Described herein is a sensitive and quantitative method for Ψ detection that does not need this labelling. This approach combines the selectivity of a 10-23 DNAzyme, which can distinguish Ψ from uridine (U), with rolling circle amplification (RCA) to increase the sensitivity of the assay.
Keywords:
detection;
sensitive radiolabeling;
pseudouridine;
method;
free method;
radiolabeling free
Journal Title: Analytical biochemistry
Year Published: 2019
Link to full text (if available)
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Journal Title: Analytical biochemistry
Year Published: 2019
Link to full text (if available)
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recommendations!