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Regulation of transcription and replication by the tetrad patterns of DNA has drawn the attention of many scientists. In this note, we discuss some disparaged parameters in the study of… Click to show full abstract
Regulation of transcription and replication by the tetrad patterns of DNA has drawn the attention of many scientists. In this note, we discuss some disparaged parameters in the study of G-quadruplex structures (G4-tetrads). Besides, the implication of "destabilization as a side-effect" by these ligands on quadruplexes is explained. The lack of strict control of in vitro cell-free experiments in terms of ionic concentration, pH, epigenetic modifications, (macro)molecular crowding, and solvent effects is evident in many previous studies. The role of these factors in ligands binding and their possible effects in G-quadruplex structures are also represented.
Journal Title: Analytical biochemistry
Year Published: 2020
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