LAUSR

Sample components can not only be changed but also activated or inactivated via enzymatic or metabolic reactions. Identifying such activity conversions is the challenge. Up to now, the instrumental complexity and required bioanalytical effort have hindered progress in understanding such mechanisms. The newly developed all-in-one nanoGIT+active system substantially extends, and at the same time, miniaturizes the state-of-the-art technology. It combines all relevant steps on the same adsorbent surface. On-surface metabolization, immediate separation, multi-imaging, and effect-directed detection is performed as all-in-one system. Such a compact, miniaturized nanomolar system was exemplarily developed for well-known food systems. The proof-of-concept showed not only the miniaturized simulation of the coordinated processes of all digestion segments, but also bioactivity changes. The results presented as images were easily understandable. The conversions on surface were verified by comparison with state-of-the-art in vitro assays (analyzed by the same method). With new bioanalytical tools, new insights will open up. Activity changes and effects on metabolization pathways can now be identified more comprehensively and cost-/time-efficiently, if compared to status quo procedures (requiring in vitro digestion assays, column chromatographic separation systems, multiple detectors and in vitro effect-directed assays). This is attractive for nutritional, health and pharmaceutical science, drug development, medicinal research and for lean laboratories.