LAUSR

Diffusion-order NMR spectroscopy (DOSY) presents a powerful tool for studying solution mixtures by recording diffusion coefficients of individual components and separating their signals into respective 1D NMR spectra. Existing DOSY experiments, however, are generally unsuitable for measurements under adverse magnetic field conditions, because calculations for diffusion coefficients strictly rely on resolved resonances in the 1D NMR spectral domain. Herein, we propose a general DOSY method by introducing intermolecular zero-quantum coherence mechanism into molecular diffusion evolution to overcome the challenge of magnetic field inhomogeneity and to record high-resolution DOSY spectra free of magnetic field inhomogeneity. Our experimental results and theoretical interpretation suggest that the proposed method allows to diffusion analysis and component discrimination on solution mixtures under externally inhomogeneous magnetic field conditions and on intact biological tissues with field inhomogeneity internally caused by magnetic susceptibility variations. This study provides a previously unreported NMR protocol for high-resolution DOSY measurements in inhomogeneous magnetic fields, thus broadening the scope of DOSY applications and showing the promising prospect for studies on chemical and biological mixture samples.