LAUSR

Cattle are the intermediate hosts for five Sarcocystis species including S. hominis and S. heydorni, which also infect humans. To investigate the prevalence of Sarcocystis infections in beef products from 17 cities in the Henan Province of central China, 62 raw beef samples from markets were collected and analyzed for Sarcocystis presence via muscle squashing microscopic observation, histological section examination, and molecular characterization with 18S rRNA gene sequencing. Sarcocystis were detected in a total of 20 of the meat samples. Four species were identified that comprised S. cruzi, S. rommeli, S. heydorni, and S. hirsuta, with S. cruzi as the dominant species. In addition, seven of the 20 infected samples were infected with two or three species. Analysis of the 18S rRNA sequences recovered from these samples suggested very little genetic diversity within each species. This study represents the first molecular identification of Sarcocystis species infection in retail beef products from China. These findings will provide valuable information for evaluating the potential public health risk of bovine Sarcocystis species infections and the control of sarcocystosis in cattle.