LAUSR

thermo-responsive cell culture dishes (UpCell ) at a concentration of 3.0 x10 cells/dish. These dishes have a polymer at the bottom that changes its characteristic with fluctuation in temperature. At 37 C the polymer is hydrophobic, the seeded cells will attach to its surface and multiply to confluence of cells. At 20 C the polymer becomes hydrophilic, detaching the cells from its surface in the form of a SCS. In this process, an adhesive extracellular matrix forms on the basal side of the cell sheets. The now floating SCS are scooped using a thin plastic square sheet, which is used to transport the SCS to the surgical field (Figure 1). SCS cells are labeled with a fluorescent marker to allow them to be identified after transplantation. After hysterotomy repair in rat models, the SCS is transplanted on the surface of the hysterotomy by sliding it down from the plastic sheet with forceps. The shape and adhesive extracellular matrix of SCS allows it to be fixated directly onto the hysterotomy without any scaffolds or sutures. Proper positioning and location of the SCS is confirmed by fluorescent microscopy and evaluated on days one, three, and seven after surgery. RESULTS: A total of 3 SCS were created from one vial of stem cell suspension, with surface areas of approximately 1cm (Figure1). All sheets were successfully transplanted in 3 rats that underwent hysterotomy and repair. In the follow up period, we were able to localize the SCS in healed rat uteruses in the transplanted location (Figure 2). CONCLUSION: Stem cell laying in sheet form is a feasible and reproducible technology in which the transplantation of stem cells can be made directly to the required location.