LAUSR

Abstract Using copepod nauplii as live feed in aquaculture hatcheries could solve high mortality rates of first-feeding fish larvae due to malnutrition. However, implementing the use of copepod nauplii on an intensive production scale requires a stable production at preferably high densities, which is problematic for calanoid copepod species like Acartia tonsa . In the present study, we evaluated the response of copepods experiencing stress under high-density conditions by assessing the acute stress level of A. tonsa . Control density was at 100 ind. L −1 while the treatments were increased stepwise up to 10,000 ind. L −1 . Three biological/physiological end-points were studied: swimming behavior, respiration rate and expression level of stress-related genes. None of the elevated densities caused any significant change in swimming behavior, respiration rate or gene expression level. This study suggests that adults of A. tonsa do not exhibit any measurable acute stress response when exposed to high culture densities for 12 h.