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Abstract Pinctada martensii is one of several important pearl oysters. Ammonia is typically added to the seawater and used during artificial reproduction to improve the fertilization rate. However, the optimal… Click to show full abstract
Abstract Pinctada martensii is one of several important pearl oysters. Ammonia is typically added to the seawater and used during artificial reproduction to improve the fertilization rate. However, the optimal ammonia concentration has not been determined. To determine the optimal ammonia concentration for fertilization success, the effect of the ammonia concentration on the fertilization rate in P. martensii was investigated under the simultaneous influence of temperature and salinity. A Box-Behnken design and response surface method were used in this study. The results showed that the ammonia concentration had a significant effect, which varied quadratically with the ammonia concentration in the ranges of the factor regimes (P
Journal Title: Aquaculture
Year Published: 2019
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