Abstract To determine the effect of vitamin C (ascorbic acid, VC) on proximate composition, muscle indexes and fatty acids profiles, triplicate groups of 30 juvenile grass carp (Ctenopharyngodon idella) were… Click to show full abstract
Abstract To determine the effect of vitamin C (ascorbic acid, VC) on proximate composition, muscle indexes and fatty acids profiles, triplicate groups of 30 juvenile grass carp (Ctenopharyngodon idella) were fed six experimental diets with graded levels of VC in a recirculating rearing system for 8 weeks. The results showed that liver moisture and fiber length were decreased with VC supplementation. Moreover, trend line analysis showed liver unsaturated fatty acids decreased at first and then increased along with dietary VC increasement. However, VC showed an inverse effect in muscle. Furthermore, histogram and trend line analysis showed ACC mRNA was in line with unsaturated fatty acids in both tissues, but CPT1 and leptin mRNA were inverse. Besides, PPAR-α, LPL and FAS mRNA abundance declined first and rose then in liver and muscle. Taken together, our data indicated that VC not only decreased liver moisture and fiber length, but also regulated liver and muscle unsaturated fatty acids content in a feedback way through fatty acid synthesis and metabolism related genes.
               
Click one of the above tabs to view related content.