Abstract Five separate feeding trials were conducted with juvenile hybrid striped bass to evaluate the effects of dietary adenosine 5′-monophosphate (AMP) on growth performance, innate immunity, digestibility, digestive enzyme activity,… Click to show full abstract
Abstract Five separate feeding trials were conducted with juvenile hybrid striped bass to evaluate the effects of dietary adenosine 5′-monophosphate (AMP) on growth performance, innate immunity, digestibility, digestive enzyme activity, and physiological responses after acute and chronic stress challenges. For all trials, a basal diet was formulated principally from soybean meal, soy protein concentrate, and fishmeal to contain 40% crude protein, 10% lipid, and 2.5 kcal estimated digestible energy g−1. The experimental diets were prepared for the dose-response trial by supplementing AMP to the basal diet at six graded levels (0, 0.25, 0.5, 0.75, 1.5 and 2.0% of dry weight) at the expense of cellulose. Based on weight gain and protein retention responses after 8 weeks of feeding, the minimum requirement was estimated in a two-slope broken line model to be 0.46% and 0.34% of dry diet, respectively. Therefore, a diet supplemented with 0.5% AMP was evaluated against the basal dietary treatment in the four follow-up trials. The subsequent digestibility trial (4-week) showed that dietary AMP at 0.5% significantly (P
               
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