LAUSR

Multi-walled carbon nanotubes (MWCNTs) are one of the most important carbon Nanoparticles (NPs). The production and use of these NPs are increasing rapidly and, therefore, the need to assess their presence in the environment and associated risks has become of prime importance. Recent studies demonstrated the impacts of different NPs on bivalves, a taxonomic group where species tolerance to anthropogenic stressors, such as pollutants, is widely variable. The Manila clam Ruditapes philippinarum is one of the most commonly used bivalve species in environmental monitoring studies and ecotoxicology tests, however, to our knowledge, no information is available on biochemical alterations on this species due to MWCNTs exposure. Thus, the present study aimed to assess the toxic effects of different MWCNT concentrations (0.01; 0.10 and 1.00mg/L) in R. philippinarum biochemical (energy reserves, metabolic capacity, oxidative status and neurotoxicity) performance, after 28days of exposure. The results obtained revealed that exposure to MWCNTs altered energy-related responses, with higher metabolic capacity and lower glycogen and protein concentrations in clams exposed to these carbon NPs. Moreover, R. philippinarum exposed to MWCNTs showed oxidative stress expressed in higher lipid peroxidation and lower ratio between reduced and oxidized glutathione, despite the activation of defence mechanisms in exposed clams. Additionally, neurotoxicity was observed by inhibition of cholinesterases activity in organisms exposed to MWCNTs. The present study provides valuable information regarding how these emerging pollutans could become a potential risk for the environment and living organisms.