LAUSR

Temperature affects physiological processes in organisms and the toxicity of chemicals. The widespread industrial use of ZnO causes contamination in aquatic ecosystems. This study aimed to investigate the chronic toxicity of ZnO at different temperatures using Daphnia magna as a model organism. The chronic toxicity of five different concentrations of ZnO was assessed at 23 °C and 28 °C. The results showed that higher concentrations of ZnO inhibited growth, production of first clutch eggs and juvenile accumulation at both 23 °C and 28 °C. Growth rate, numbers of first clutch eggs and juvenile accumulation were lower at 28 °C than at 23 °C. We also observed the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. At higher concentrations of ZnO, oxidative stress was induced leading to increase MDA level and decrease SOD activity at 28 °C. These findings indicated that high temperature and high concentration of ZnO inhibited the activity of enzymatic proteins. Nonetheless, among all treatments, the accumulation of zinc in D. magna was not significantly different. Our results suggested that both ZnO and higher temperature induced oxidative stress in D. magna. As a result, MDA concentration increased, SOD activity changed and the growth and reproduction of D. magna was adversely affected.