LAUSR

Abstract A Novel class of 1,4-disubstituted 1,2,3-triazoles have been synthesized in good to excellent yields via Cu(I) accelerated azide-alkyne click chemistry reaction strategy. The newly synthesized compounds were assessed for their in vitro antimicrobial activity against five Gram-positive, seven Gram-negative bacteria and three fungi. Most of the synthesized compounds displayed significant activity against the tested Gram-positive and Gram-negative bacteria. Molecular docking study revealed that all docked compounds are bound efficiently with the active site of Topoisomerase IV (4EMV) receptor with the observed the free energy of binding from -7.79 to -9.44 kcal/mol. Interestingly, compound 13a forms four hydrogen bonds and displayed high binding energy (-9.44 kcal/mol) with the Topoisomerase IV (4EMV) receptor which correlated with their in vitro antimicrobial assays. ______________________________________________________________________________Keywords: 1,4-Disusbstituted-1,2,3-triazoles, Click chemistry, antimicrobial activity; Docking study