LAUSR

Abstract Malignant melanoma is a lethal human skin cancer that is not easily treatable through traditional medicines, surgeries, and therapies. Millions of cases are recorded annually to cure physiological skin defects by chemotherapy that causes several adverse effects and challenges. Moreover, pathogenic infections might aggravate infection with subsequent ulceration or cutaneous melanoma. Accordingly, we synthesized nanodrug by loading chemotherapeutic agent, 5-Fluorouracil (FU-5), onto the reduced graphene oxide (rGO). We then extracted arabinoxylan (ARX) from the husk of Plantago Ovata and functionalized it into carboxymethylarabinoxylan (CMARX) and loaded synthesized nanodrug. We have crosslinked CMARX/nanodrug with different tetraethylorthosilicate (TEOS) into nanocomposite hydrogel rGO-FU5-CMARX system melanoma skin cancer treatment. These nanocomposite hydrogel systems rGO-FU5-CMARX have exhibited different physicochemical properties. These properties were analyzed through FTIR, SEM, water contact angle, swelling in different media (aqueous and PBS) and biodegradation in PBS media. The in-vitro activities, i.e., drug delivery via Franz diffusion, antibacterial against S. aureus and P. aeruginosa and the anticancer activities was performed against Uppsala 87 Malignant Glioma (U-87) cell lines. Moreover, rGO-FU5-CMARX hydrogel nanocomposites displayed different antimicrobial and anticancer activities based on different crosslinking. Hence, an inventive Graphene oxide–Carboxymethylarabinoxylan based nanocomposite hydrogel drug-delivery system was developed to treat skin cancer after bacterial infections.