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s 75 Conclusions: ob/ob mice presented a premature cardiac dysfunction without a further age-related deterioration and a reduction in the abdominal aorta and carotid artery mean diameter in adult age. P79 ULTRASONOGRAPHIC CHARACTERIZATION OF THE db/db MOUSE, AN ANIMAL MODEL OF METABOLIC ABNORMALITIES Nicole Di Lascio , Chiara Rossi , Claudia Kusmic , Anna Solini , Francesco Faita 2 Department of Clinical and Experimental Medicine, Italy Institute of Clinical Physiology, CNR, Pisa, Italy Department of Surgical, Medical, Molecular and Critical Area Pathology, University of Pisa, Pisa, Italy Purpose/Background/Objectives: The availability of an animal model that, beside common blood parameters like hyperglycemia or high lipid levels, reliably mirrors organ damage occurring in metabolic diseases, is an urgent need. These animals have not been fully characterized in terms of cardiovascular, renal and hepatic ultrasound parameters, and only sparse and discordant values can be found in literature. Aim of this paper is to provide a detailed, non invasive description of heart, vessels, liver and kidneys of the db/db mouse by ultrasound imaging. Methods: 16 wild type and 34 db/db 11 week-old male mice were studied. State of the art ultrasound technology was used to acquire images of cardiovascular, renal and hepatic districts. A full set of parameters describing function of the selected organs was evaluated. Results: db/db mice are characterized by systolic and diastolic dysfunction, confirmed by strain analysis. On the contrary, abdominal aortic and carotid stiffness seem to be not increased in these diabetic rodents; furthermore, they are characterized by a smaller mean diameter for both vessels. Renal microcirculation is significantly compromised, while liver steatosis is only borderline higher in db/db mice than in control animals. Conclusions: We offer here for the first time an in vivo detailed ultrasonographic characterization of the db/db mouse model, providing a useful tool for a thoughtful choice of the right rodent model for any experimental design. P80 IDENTIFICATION OF RADIAL VASCULAR WALL ABNORMALITIES BY VERYHIGH FREQUENCY ULTRASOUND IN PATIENTS WITH FIBROMUSCULAR DYSPLASIA: THE FUCHSIA STUDY Rosa Maria Bruno , Nicole Di Lascio , Daniela Guarino , Saverio Vitali , Piercarlo Rossi , Davide Caramella , Stephane Laurent , Pierre Boutouryie , Stefano Taddei , Lorenzo Ghiadoni , Francesco Faita 2 University of Pisa, Pisa, Italy Institute of Clinical Physiology e CNR, Pisa, Italy Hôpital Européen Georges Pompidou, INSERM, Paris, France Aim: This case-control study is aimed at identifying radial vascular wall abnormalities in patients with fibromuscular dysplasia (FMD). Methods: High-frequency ultrasound scans of radial arteries were obtained by VevoMD (70MHz probe, FUJIFILM, VisualSonics). Radial wall showed two echogenic interfaces: the 1 (lumen-media) and the 2 (media-adventitia). Intima-media (IMT), adventitia (AT), and global thickness (IMAT) and wall cross-sectional area (WCSA) Measured. Vascular wall disarray was assessed calculating the root mean square error (RMSE) between 20 gray-level profiles crossing the two interfaces and the profile obtained averaging them, normalized for the maximum value of the corresponding mean profile (RMSE/mean). Results: 11 treated hypertensive female FMD patients and 8 healthy controls (C) were enrolled (age 52 5 vs 45 13years, pZ 0.51; BMI 26 3 vs 23 3kg/mg, pZ 0.12; mean BP 97 7 vs 85 10 mmHg, pZ 0.01). Radial lumen was similar; IMT (0.166 0.037 vs 0.128 0.022mm, pZ 0.03), AT (0.114 0.029 vs 0.083 0.019mm, pZ 0.008) and IMAT (0.281 0.042 vs 0.211 0.027mm, pZ 0.003) were higher in FMD. Wall/lumen ratio was similar and WCSA increased in FMD. The maximum values of 1 (121 43 vs 157 22, pZ 0.09) and 2 interface 109 44 vs133 18, pZ 0.09) tended to be lower, whereas RMSE/mean was higher in FMD (1 1.31 0.24 vs 0.83 0.32, pZ 0.006; 2 1.37 0.38 vs 0.94 0.32, pZ 0.03). The difference was attenuated for the 1 but not for the 2 interface when considering age and mean BP as covariates (pZ 0.054 and pZ 0.016 respectively). Conclusions: The radial artery wall of hypertensive FMD patients shows increased thickness and its ultrastructure is characterized of loss of echogenicity and inhomogeneity of the two echogenic layers. P81 DISARRAY AND REMODELING OF THE RADIAL ARTERY IN WOMEN WITH SPONTANEOUS CORONARY ARTERY DISSECTION: THE FUCHSIA STUDY Rosa Maria Bruno , Nicole Di Lascio , Abtehale Al Hussaini , Daniela Guarino , Saverio Vitali , Piercarlo Rossi , Davide Caramella , Bernardo Cortese , Francesco Faita , Stefano Taddei , Lorenzo Ghiadoni , David Adlam 3 University of Pisa, Pisa, Italy Institute of Clinical Physiology e CNR, Pisa, Italy University of Leicester, Leicester, UK ASST Fatebenefratelli-Sacco, Milano, Italy Background: Spontaneous coronary dissection (SCAD) may represent a manifestation of fibromuscular dysplasia (FMD); thus, preclinical lesions might be found in extracoronary vessels with similar size and wall ultrastructure, such as the radial artery. Methods: Two 5’-clips from the left radial artery were obtained by Vevo MD (70 MHz probe, FUJIFILM, VisualSonics). Radial wall showed two echogenic interfaces: the 1st (lumen-media) and the 2nd (media-adventitia). Intimamedia (IMT), adventitia (AT), and global thickness (IMAT) and wall crosssectional area (WCSA) Measured. Vascular wall disarray was assessed calculating the root mean square error (RMSE) between 20 gray-level profiles crossing the two interfaces and the profile obtained averaging them, normalized for the maximum value of the corresponding mean profile (RMSE/mean).