LAUSR

Abstract The aim of current study is to explore the biosynthesis of silver nanoparticles (AgNPs) mediated by silver tolerant bacteria (AgTB) and its ameliorative efficacy against ESKAPE pathogens under in vitro condition. In present investigations, we have observed a metallic AgNPs from extracellular regimen via reduction of aqueous Ag+ events by using AgNO3 salt tolerated bacteria. This cascade was considered as potential candidates for the rapid synthesis of such AgNPs. In addition, the protective potential of AgNPs was evaluated with different procedures as MIC, MBC, and antibiofilm action against ESKAPE pathogen. In our results, AgTB identified as Bacillus cereus and displayed a well marked rapid synthesis of AgNPs within 3 h. The AgNPs was evaluated by using UV–vis spectroscopy and XRD. The DLS were applied to quantify the size distribution profile of AgNPs. And also, morphological were determined by using TEM study. In our result, TEM images exhibited well defined shape and size with an average particle size of about 17.51 nm. Likewise, FTIR analyses were shown marked validation with secondary metabolites which may bound to AgNPs and contributed for their stability. Our screened AgNPs showed both anti–microbial and anti–biofilm potency against multi–drug resistant ESKAPE pathogen. This bacterial mediated synthesis may be considered as an alternative approach for traditional chemical (toxic) and physical (high energy expensive) synthesis methods; and ultimately these nanoparticles can be applied for the treatment strategies for drug resistant ESKAPE pathogens.