LAUSR

Abstract The bio potential behavior of green silver nanoparticles (AgNPs) using Acalypha wilkesiana flowers extract weas evaluated. The AgNPs formed were confirmed using surface plasmon resonance band showed in UV–vis spectrophotometer that characterized with spherical shape in size range from 5 to 45 nm depending on Transmission electron microscopy (TEM) and with good stability (−50.75 mV) depending on Zeta-potential measurements. In order to identify the capping agents responsible for the reduction of silver ion and the stabilization of nanoparticles prepared, Fourier transform-infrared spectroscopy (FTIR) and HPLC-ESI-MS analysis were used. Our AgNPs exhibited cytotoxic activity against MCF-7 (Breast carcinoma), PC-3 (Prostate carcinoma) and BJ-1 (skin normal human) cell lines with IC 50 values 4.00, 3.6 and 4.1 μg/mL, respectively (i.e. the higher selectivity of AgNPs toward prostate than breast cancer cell lines was observed). These highly cytotoxic activity may be back to the presence of the bi-apigenin derivatives; amentoflavone and Cupressuflavone that displayed unique binding docking score towards the active site of human DNA topoisomerase enzyme. The present study is a distinctive application for the biosynthesis of silver nanoparticles in a fast, simple, easy, eco-friendly, inexpensive and effective way.