LAUSR

Abstract Alternative sources for weed control are necessary to reduce the use of synthetic herbicides. Bioherbicides obtained from agro-industrial waste have the potential to integrate sustainable management of existing agricultural systems. Because of the need for new compounds with potential herbicide, this study's objective was to evaluate the bioherbicidal effect and the enzymatic activity of the extracts produced using agro-industrial waste as a substrate. A submerged fermentation was carried out with orange peel and shrimp shell; Trichoderma koningiopsis and Rhizopus stolonifer were used separately to produce enzymatic extracts. Through submerged fermentation with these agro-industrial residues, extracts were obtained, which were characterized concerning the activities of pectinase, cellulase, amylase, laccase, and peroxidase. The extracts were also evaluated for their bioherbicidal potential for weed control, and this is the first study conducted with these residues for this purpose. No significant difference was observed in the enzymatic activities of the extracts produced by the different microorganisms used. Accentuated leaf damage was observed in C. sativus seven days after applying both extracts, ranging from 66.67 to 77.08% for extracts of T. koningiopsis and R. stolonifer, respectively. Therefore, the extracts appear to be promising alternatives in weed control just seven days after application, demonstrating a rapid effect with low environmental risk.