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An image analysis method to quantify heterogeneous filamentous biomass based on pixel intensity values – Interrelation of macro- and micro-morphology in Actinomadura namibiensis

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Abstract The cell morphology of the filamentous actinobacterium Actinomadura namibiensis was evaluated by image analysis of microscopic pictures. A pixel intensity thresholding method was used to differentiate between pelleted bioagglomerates,… Click to show full abstract

Abstract The cell morphology of the filamentous actinobacterium Actinomadura namibiensis was evaluated by image analysis of microscopic pictures. A pixel intensity thresholding method was used to differentiate between pelleted bioagglomerates, the macro-morphology, and the characterization of individual hyphae, the micro-morphology. At both scales morphological differences between salt-enhanced cultivation (addition of 50 mM (NH4)2SO4), which increased the labyrinthopeptin A1 concentration from

Keywords: actinomadura namibiensis; pixel intensity; micro morphology; morphology; image analysis

Journal Title: Biochemical Engineering Journal
Year Published: 2020

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