Abstract Crude extracts of the fungus Metarhizium guizhouense PSUM02 grown on protein- enriched medium showed high proteolytic activity and produced one main protein indicated as fungal protease with 33 kDa molecular… Click to show full abstract
Abstract Crude extracts of the fungus Metarhizium guizhouense PSUM02 grown on protein- enriched medium showed high proteolytic activity and produced one main protein indicated as fungal protease with 33 kDa molecular mass. The protease activity had high stability when incubated at 10 or −20 °C for 6 months. The use of Metarhizium spores (1 × 104 spore mL−1) in combination with its protease (354.1 ± 2.2 U mL−1) completely inhibited hatching of the root-knot nematode Meloidogyne incognita within 24 h. Moreover, the mortality of juveniles increased to 100% at 48 h post treatment. The results were significantly different (p
               
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