The purpose of this study was to investigate fungal diversity and relative abundance (RA) during pig manure composting via high-throughput sequencing approach. Fine coal gasification slag (FCGS) (0%, 2%, 4%,… Click to show full abstract
The purpose of this study was to investigate fungal diversity and relative abundance (RA) during pig manure composting via high-throughput sequencing approach. Fine coal gasification slag (FCGS) (0%, 2%, 4%, 6%, 8% and 10%) were added into composting raw materials as additive and performed 42 days. Adjust C/N and moisture to 30 and 65%. Results showed that dominant phyla were Ascomycota (99.62%) and Basidiomycota (0.38%). The main genera were Epicoccum (1.26%), Alternaria (83.35%), Aspergillus (12.08%) and Gibberella (1.69%). 10% treatment got the higher abundance and operational taxonomic units number from rank abundance curve and petals diagram. Compared with control, FCGS amendment composting could increase the sanitary time (3-7 d) and total nitrogen (0.05-12.03%). The principal component analysis was considered that FCGS treatments and control had significantly difference. The RA of fungi varied among all treatments. Therefore, 10% treatment was a potential candidate to enhance fungal diversity and composting quality.
               
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