LAUSR

Abstract Phytases are phosphatases employed in monogastric animal feed to improve animal growth rates and protect the environment by reducing phosphorous in manure. In this study, the highly active phytase PhyAsr of the protein tyrosine phosphatase class, which was derived from the ruminal bacterium Selenomonas ruminantium, was successfully produced for the first time in the chloroplast of the green microalga Chlamydomonas reinhardtii. Two homoplasmic lines (C1 and C3) exhibited the highest level of PhyAsr expression, while the highest phytase activity was detected in the C1 line at 34 U/g dry weight. The results of this study indicate that C. reinhardtii is a suitable host for producing the phytase PhyAsr, which may be utilized as a supplement in the monogastric animal diet.