LAUSR

Giant plasma membrane vesicles (GPMVs) are unilamellar bodies that can be easily isolated from mammalian cells. SNAP-tag is a protein tag which binds covalently with benzylguanine (BG) derivatives, and is also capable of being fused to a protein of interest. Currently, specifically anchoring cellular material remains a challenge for bioengineering. The purpose of our project was to create a benzylguanine-covered surface to immobilize GPMVs expressing SNAP-tag fused to A2A, a G-protein coupled receptor. The ability to anchor vesicles from mammalian cells could be instrumental for the development of functional biosensors. Using this novel anchoring system, a wide variety of compounds could be quickly detected via binding to an exogenously expressed protein of interest within the same membrane environment. SNAP-tag fused A2A protein successfully expressed in HEK cells